Stromalab - Targeting the stroma to regenerate Médecine régénératrice, thérapie cellulaire, cellules souches mésenchymateuses, tissu adipeux, moelle osseuse, réparation, régénération Regenerative medicine, cell therapy, mesenchymal stem cells, adipose tissue, bone marrow, repair, regeneration

Multiphoton spectral microscope

cper-occitanie

Recent advances in optical imaging open up new possibilities for studying biological tissues with subcellular resolution. Genetically modified mice are now valuable tools for in vivo cell monitoring thanks to the expression of several fluorescent proteins in the same animal. These fluorescent molecules injected or produced by a genetically modified organism target a specific organ, cell type, or molecule, which will thus be highlighted by imaging.
Thanks to fluorescence, researchers can observe tissues in vivo and better understand the development of certain pathologies or migratory phenomena involved in tissue repair and/or regeneration processes. However, they face the problem of auto fluorescence, i.e. the spontaneous fluorescence of certain biomolecules.
Stromalab recently acquired an AxioExaminer LSM 880 Zeiss biphotonics microscope. This material allows 3D and 4D imaging in vivo in small animals. This microscope, thanks to QUASAR technology, offers the great advantage of being able to perform simultaneous spectral detection in a single scan. Thus, in a single acquisition, without loss of time and without space-time lag, it is possible to acquire and perfectly separate more than 4 fluorochromes whose spectra overlap. This technology also eliminates the need for the auto fluorescence signal generated by tissues. The NLO system also provides access to the second harmonic generation signal (SHG), making it possible to reveal, in particular, the organization of collagen fibres in the extracellular matrix or the striations of skeletal muscles without using any marking.

 

Corinne Barreau

PhD, IE UPS

corinne.barreau@inserm.fr

Tél : 05 34 60 95 14

Microscope Zeiss LSM 880

  • Right stand (AxioExaminer). Maximum distance under the lens: 11 cm
  • XY motorized board
  • 4 position lens turret with only one lens in low position for observation => Clear space around the sample
  • Linear scanner at 13 f/s
  • QUASAR spectral detection system with 2 lateral Alkaline PMTs and 1 central GaAsP spectral detector composed of 32 high sensitivity detectors, allowing :
    • acquisition of up to 10 spot channels including 8 GaAsP
    • simultaneous spectral acquisition from 391 to 749 nm
    • free configuration of the fluorescence emission window
  • For single-photon imaging: laser 458, 488, 514, 561 and 633 nm
  • For biphoton imaging: Chameleon precompensated system
  • Image acquisition and analysis software package: ZEN Black, ZEN Blue, IMARIS

 

Image1

 

Intravital imaging module

  • TEM SEGA gas anaesthesia system, equipped with Tec7 isoflurane evaporator.
  • Carrier gases: air and oxygen
  • Monitoring system for Physiosuite rodents (Kent Scientific) :
    • Respirator
    • Temperature control
    • Pulse oximeter
    • Trigger function (can be controlled by the acquisition software)

 

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