Hypoxia is defined as the reduction or absence of oxygen in the organs, tissues or cells. This hypoxia may be physiological (in bone marrow, adipose tissue ….) or pathological (cancer, inflammatory diseases, diabetes, ischemia …). The accurate control of oxygen levels in cells cultures is essential to obtain reproducible and consistent results. However with conventional incubators and hoods, regulation of low oxygen level in vitro is difficult to control and maintain long-term. To mimic physiological oxygenation conditions, the laboratory STROMALab has acquired an innovative closed cultivation system controlled atmospheres XVivo (Biospherix).
The most important advantage of this system is that the parameters monitored in the incubators are also controlled in process chamber. Moreover all these parameters can be kept stable throughout the culture. Thus the cells will not suffer any environmental disturbance except those defined by the experimenter. The XVivo system could be applied to all types of cells.
Availability of XVivo system
The XVivo system is open to the scientific community of the Occitanie region after contact and discussion with those responsible for this equipment.
Unlike conventional cell culture systems, the XVivo system is a completely closed system, consisting of hoods and incubators with observation system. This is a modular system that allows a wide variety of possible combinations. The Xvivo system acquired by STROMALab consists of:
– two modules with one process chamber and three independent incubators;
– one module containing a microscope Nikon DS-Fi2.
These modules system are totally independent allowing to control all parameters independently. The main parameters controlled that can be modulated according to user requirements are:
– oxygen level
– carbon dioxide level
– nitrogen level