STROMALab Médecine régénératrice, thérapie cellulaire, cellules souches mésenchymateuses, tissu adipeux, moelle osseuse, réparation, régénération Regenerative medicine, cell therapy, mesenchymal stem cells, adipose tissue, bone marrow, repair, regeneration

Hypoxia XVivo

Hypoxia is defined as the reduction or absence of oxygen in the organs, tissues or cells. This hypoxia may be physiological (in bone marrow, adipose tissue ….) or pathological (cancer, inflammatory diseases, diabetes, ischemia …). The accurate control of oxygen levels in cells cultures is essential to obtain reproducible and consistent results. However with conventional incubators and hoods, regulation of low oxygen level in vitro is difficult to control and maintain long-term. To mimic physiological oxygenation conditions, the laboratory STROMALab has acquired an innovative closed cultivation system controlled atmospheres XVivo (Biospherix).
The most important advantage of this system is that the parameters monitored in the incubators are also controlled in process chamber. Moreover all these parameters can be kept stable throughout the culture. Thus the cells will not suffer any environmental disturbance except those defined by the experimenter. The XVivo system could be applied to all types of cells.

Availability of XVivo system

The XVivo system is open to the scientific community of the Occitanie region after contact and discussion with those responsible for this equipment.

Isabelle ADER

PhD, CR1 Inserm

isabelle.ader-perarnau@inserm.fr

+33 5 61 28 37 74    

Marie-Laure RENOUD

Assistant Ingénieur Inserm

marie-laure.renoud@inserm.fr

+33 5 61 28 37 23

Unlike conventional cell culture systems, the XVivo system is a completely closed system, consisting of hoods and incubators with observation system. This is a modular system that allows a wide variety of possible combinations. The Xvivo system acquired by STROMALab consists of:
– two modules with one process chamber and three independent incubators;
– one module containing a microscope Nikon DS-Fi2.
These modules system are totally independent allowing to control all parameters independently. The main parameters controlled that can be modulated according to user requirements are:
– temperature ;
– oxygen level;
– carbon dioxide level;
– nitrogen level;
– humidity.

Enceinte hypoxie XVivo

STROMALAB

CHU TOULOUSE – HÔPITAL DE RANGUEIL

Bât L1, 2ème étage

1 avenue Jean Poulhès – BP 84225

31432 TOULOUSE CEDEX 4

Phone : +33 5 61 28 37 21

Contact us

Actuality

Loïc FIEVET wins the oral communication award at the 17th SFGM-TC Congress

20 November 2017 |  

Our Doctoral student Loïc Fiévet was awarded the prize for oral communication at the 17th SFGM-TC Congress - Société Francophone de...


Computational Epigenomics Tools to Understand the Cellular Language of Pluripotency and Reprogramming

12 October 2017 |  

Dr. Marcos Arauzo-Bravo, Director of the bioinformatics platform of the bio-medical research institute of "Donostia" (San Sebastián), will present what he...


Human bone regeneration conference

12 October 2017 |  

Dr. Pierre Layrolle, Director of INSERM 1238 "Phy-OS" of the Faculty of Medicine of of Nantes, will be coming to give...

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