Head : Dr. Frédéric DESCHASEAUX
The main tissues of mesenchymal stem / stromal cells (MSCs) are bone marrow (BM) and adipose tissue (AT). These cells together form a network of cells ranging from the stem cell to the progenitors to the differentiated cells that are osteoblasts, chondrocytes, perivascular cells and medullary adipocytes for bone marrow, CSMs and adipocytes, perivascular cells and fibroblasts for TA MSCs. In humans, the identities and microenvironments of stem cells and progenitors remain to be discovered.
Scientific objective :
The objectives of the group are therefore to characterize these subpopulations of CSM within the MO or TA in adult humans. To do this, we use culture technologies in different O2 environments (normoxia vs. hypoxia) that are as close as possible to physiological environments. We have also developed novel 3D culture approaches that provide cells with microenvironments similar to those in vivo. We use cell sorting and confocal microscopy techniques to work on rare populations that we can then exhaustively analyze through their entire transcriptome or miRNome.
Expected health benefits :
Understand the cellular heterogeneity that initiate the formation of tissues such as human MO makes it possible to prevent or propose new therapeutic approaches to pathologies associated with hematopoietic cells, bone tissue or cartilage. At the level of the TA, the knowledge acquired could bring here also solutions to fight against the obesity and the metabolic pathologies which are associated there.
See organizational chart here