In order to analyse the gene expression differences between physiological and pathological states, we use different approaches :
via qPCR (96 wells)
via pangenomic array hybridation (Affymetrix)
Gene expression may be also addressed via other technologies on the platform GeT Genotoul.
Measure nucleic acid concentrations
- Nanodrop 2000 (Thermo Scientifique) : spectrophotometer UV/visible
qPCR
- StepOnePlus™ Real-Time PCR System (Applied Biosystem)
- CFX96™Real-Time PCR Detection System (Biorad)
Microarrays
- GeneAtlas® Personal Microarray System (Affymetrix)
For array strip