Stromalab - Targeting the stroma to regenerate Médecine régénératrice, thérapie cellulaire, cellules souches mésenchymateuses, tissu adipeux, moelle osseuse, réparation, régénération Regenerative medicine, cell therapy, mesenchymal stem cells, adipose tissue, bone marrow, repair, regeneration

Analyse of transcriptome

In order to analyse the gene expression differences between physiological and pathological states, we use different approaches :

via qPCR (96 wells)

via pangenomic array hybridation (Affymetrix)

Gene expression may be also addressed via other technologies on the platform GeT Genotoul.

http://get.genotoul.fr/

Emmanuelle Arnaud

IR CNRS

emmanuelle.arnaud@inserm.fr

Tél : +33 5 61 28 37 74

Measure nucleic acid concentrations

  • Nanodrop 2000 (Thermo Scientifique) : spectrophotometer UV/visiblenanodrop-2000

 

qPCRcfx96stepone-plus

  • StepOnePlus™ Real-Time PCR System (Applied Biosystem)
  • CFX96™Real-Time PCR Detection System (Biorad)

 

Microarrays

gene-atlas-affymetrix

  • GeneAtlas® Personal Microarray System (Affymetrix)

For array strip