STROMALab Médecine régénératrice, thérapie cellulaire, cellules souches mésenchymateuses, tissu adipeux, moelle osseuse, réparation, régénération Regenerative medicine, cell therapy, mesenchymal stem cells, adipose tissue, bone marrow, repair, regeneration

Analyse of transcriptome

In order to analyse the gene expression differences between physiological and pathological states, we use different approaches :

via qPCR (96 wells)

via pangenomic array hybridation (Affymetrix)

Gene expression may be also addressed via other technologies on the platform GeT Genotoul.

http://get.genotoul.fr/

Emmanuelle Arnaud

IR CNRS

emmanuelle.arnaud@inserm.fr

Tél : +33 5 61 28 37 74

Measure nucleic acid concentrations

  • Nanodrop 2000 (Thermo Scientifique) : spectrophotometer UV/visiblenanodrop-2000

 

qPCRcfx96stepone-plus

  • StepOnePlus™ Real-Time PCR System (Applied Biosystem)
  • CFX96™Real-Time PCR Detection System (Biorad)

 

Microarrays

gene-atlas-affymetrix

  • GeneAtlas® Personal Microarray System (Affymetrix)

For array strip

 

STROMALAB

CHU TOULOUSE – HÔPITAL DE RANGUEIL

Bât L1, 2ème étage

1 avenue Jean Poulhès – BP 84225

31432 TOULOUSE CEDEX 4

Phone : +33 5 61 28 37 21

Contact us

Actuality

Dr Jordi Alcaraz – Head of the Translational Fibroblast Mechanobiology group; Serra-Húnter Associate Professor – University of Barcelona (Spain)

13 September 2017 |  

« Aberrant mechanobiology in cancer and fibrosis » Friday 09/17/17 at 11AM Room Hervé PARIS Building I2MC, CHU Rangueil, 31432 Toulouse,...


Dr Christian DANI – Laboratoire “Cellules Souches et Différenciation”, iBV, UMR7277 CNRS – UMR1091 INSERM, Université de Nice Sophia-Antipolis – Nice

11 September 2017 |  

« Adipose progenitors derived from human IPS cells : a new tool for cell therapy of obesity? » Friday 09/29/17 at...


Characterization of adipose and muscular stromas during regeneration in a lipodystrophic mouse model: does adipocytes need to be regenerated?

27 June 2017 |  

Tainee : GIROUSSE Amandine e-mail: amandine.girousse@inserm.fr Phone : +33-5-61-28-37-74  more information 

See all